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KMID : 0613820060160020345
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Journal of Life Science
2006 Volume.16 No. 2 p.345 ~ p.351
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Phylogenetic Diversity and Comparison of RAPD Fingerprint Profile of Streptococcus iniae
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Jeong Yong-Uk
Heo Moon-Soo
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Abstract
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Streptococcus sp. were isolated from cultured flounder (Paralichthys olivaceus) having Streptococcosis during 2004 to 2005 in Jeju Island. Ninety four Streptococcus iniae strains were isolated using biochemical test and multiplex PCR assay. Three genotypes (A, B, C-type) of S. iniae were appeared in the RAPD analysis and they showed international or local genetic polymorphism. Presently, S. iniae having A-type is a dominant S. iniae genotype in Jeju and showed band patterns at about 550, 850, 1000, 1300 and 2000 base pares. In this study, the reported P14 random primer, that used to distinguish serotypes of S. iniae could not be applied to distinguish Jeju island S. iniae’s genetic polymorphism.
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KEYWORD
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Streptococcus iniae, genotype, serotypes, genetic polymorphism
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